Assessment of Two Techniques for Diagnosis Pulmonary Tuberculosis in HIV Patient at Mnazi Mmoja Hospital Zanzibar.

Mohamed, Abdalla Said (2016) Assessment of Two Techniques for Diagnosis Pulmonary Tuberculosis in HIV Patient at Mnazi Mmoja Hospital Zanzibar. Masters thesis, The Open University of Tanzania.

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Abstract

HIV associated TB is not easy to make diagnosis and is linked with extreme disease and death. The spread of MDR-TB, together with increasing harmful of HIV infection and inadequate availability of quick examination instrument have lead to cause disappointment of global TB control. The intention of the study was to evaluate Gene Xpert MTB/RIF technique and smear auramine LED FM technique for detection of pulmonary tuberculosis (PTB) in people living with HIV at Mnazi Mmoja Hospital Zanzibar. The study was experimental type of design that involved laboratory analysis of sputum specimens for determination of Mycobacterium tuberculosis as well as rifampicin resistance for HIV patients. The specimens were processed in Gene Xpert MTB/RIF and smear auramine LED FM. The sum of 246 patients sputum specimens were analyzed for the existence of Mycobacterium tuberculosis by means of Gene Xpert MTB/RIF and LED FM techniques. The results showed that 169 (68.7%) spot samples (sample I) and 169 (68.7%) morning samples (sample II) were positive for Gene Xpert MTB/RIF technique. Then 112 (45.5%) spot samples (sample I) and 118 (48.0%) morning samples (sample II) were smear positive (LED FM). The sensitivity of LED FM in spot samples and morning samples were 66.3% and 69.8% respectively. Among 169 positive TB cases 2 patients were found with rifampicin resistance. The outcome of this study indicated that the performance of Gene Xpert MTB/RIF machine is more accuracy than LED FM in diagnosis of PTB in HIV patients suspected with TB. Gene Xpert MTB/RIF may be significant as a single quick method for PTB case finding in HIV patients suspected with active TB.

Item Type: Thesis (Masters)
Subjects: 500 Science > 570 Life sciences
Divisions: Faculty of Sciences Technology and Environmental Studies > Department of life Sciences
Depositing User: Mr Habibu V. Kazimzuri
Date Deposited: 16 Feb 2017 09:55
Last Modified: 23 May 2017 11:16
URI: http://repository.out.ac.tz/id/eprint/1545

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